Journal: Journal of Virology

Article Title: Neutrophils Play an Essential Role in Cooperation with Antibody in both Protection against and Recovery from Pulmonary Infection with Influenza Virus in Mice

doi: 10.1128/jvi.01210-07

Figure Lengend Snippet: FIG. 1. Survival rates of PMN-depleted and control mice infected with a small (1.0 102 PFU) (A) or a large (1.5 103 PFU) (B) inoculum of influenza A virus strain PR8 and receiving passive transfer of anti-PR8 antiserum or NMS (or neither) 3 days (3d) after infection. BALB/c mice (8 to 10/group) were daily injected i.p. with MAb RB6 or control rat IgG from day 2 to day 8 after virus infection. Mice were intranasally (i.n.) infected with either inoculum of PR8 on day zero.

Article Snippet: The rat IgG2b MAb RB6 was prepared from the culture supernatant and ascites of pristane-primed BALB/c nude mice transplanted with the hybridoma by affinity chromatography with protein G-agarose (Fast Flow column; Millipore Co., Billerica, MA) and was quantified using an enzyme-linked immunosorbent assay quantitation kit for rat IgG2b antibody (Bethyl Laboratories, Inc., Montgomery, TX).

Techniques: Control, Infection, Virus, Injection

Journal: Journal of Virology

Article Title: Neutrophils Play an Essential Role in Cooperation with Antibody in both Protection against and Recovery from Pulmonary Infection with Influenza Virus in Mice

doi: 10.1128/jvi.01210-07

Figure Lengend Snippet: FIG. 2. Pulmonary virus titers of mice infected with a small inoculum of influenza A virus strain PR8 (1.0 102 PFU) and effects of passive transfer of anti-PR8 or NMS into mice on the virus titers. MAb RB6-treated mice (triangles) and rat IgG-treated control mice (circles) were intranasally (i.n.) infected with PR8 virus and received passive transfer of anti-PR8 (open symbols) or NMS (solid symbols) at 1 h before (A), and on day 1 (B) and 3 (C) after, PR8 infection. Results are expressed as means SDs for 6 to 8 mice per group. Asterisks indicate that the titers in the lungs of PMN-depleted and/or anti-PR8-transferred mice are significantly different (P 0.05) from the titers in the lungs of control mice.

Article Snippet: The rat IgG2b MAb RB6 was prepared from the culture supernatant and ascites of pristane-primed BALB/c nude mice transplanted with the hybridoma by affinity chromatography with protein G-agarose (Fast Flow column; Millipore Co., Billerica, MA) and was quantified using an enzyme-linked immunosorbent assay quantitation kit for rat IgG2b antibody (Bethyl Laboratories, Inc., Montgomery, TX).

Techniques: Virus, Infection, Control

Journal: Journal of Virology

Article Title: Neutrophils Play an Essential Role in Cooperation with Antibody in both Protection against and Recovery from Pulmonary Infection with Influenza Virus in Mice

doi: 10.1128/jvi.01210-07

Figure Lengend Snippet: FIG. 3. Pulmonary virus titers of mice infected with a large inoculum of influenza A virus strain PR8 (1.5 103 PFU) and effects of passive transfer of anti-PR8 or NMS into mice on the virus titers. MAb RB6-treated mice (triangles) and rat IgG-treated control mice (circles) were intranasally (i.n.) infected with PR8 virus and received passive transfer of anti-PR8 (open symbols) or NMS (solid symbols) at 1 h before (A), and on day 1 (B) and 3 (C) after, PR8 infection. Results are expressed as means SDs for 4 to 8 mice per group. Asterisks indicate that the titers in the lungs of PMN-depleted and/or anti-PR8-transferred mice are significantly different (P 0.05) from the titers in the lungs of control mice.

Article Snippet: The rat IgG2b MAb RB6 was prepared from the culture supernatant and ascites of pristane-primed BALB/c nude mice transplanted with the hybridoma by affinity chromatography with protein G-agarose (Fast Flow column; Millipore Co., Billerica, MA) and was quantified using an enzyme-linked immunosorbent assay quantitation kit for rat IgG2b antibody (Bethyl Laboratories, Inc., Montgomery, TX).

Techniques: Virus, Infection, Control

Journal: Journal of Virology

Article Title: Neutrophils Play an Essential Role in Cooperation with Antibody in both Protection against and Recovery from Pulmonary Infection with Influenza Virus in Mice

doi: 10.1128/jvi.01210-07

Figure Lengend Snippet: FIG. 4. ND50s in the sera of mice infected with 1.0 102 PFU of influenza A virus strain PR8 before or after passive transfer of anti-PR8. Mice treated with MAb RB6 (triangles) and control mice treated with rat IgG (circles) received NMS (solid symbols) or anti-PR8 antiserum (open symbols) at 1 h before (A), and on day 1 (B) and 3 (C) after, virus infection. Results are expressed as ND50s of pooled sera from 6 to 8 mice per group.

Article Snippet: The rat IgG2b MAb RB6 was prepared from the culture supernatant and ascites of pristane-primed BALB/c nude mice transplanted with the hybridoma by affinity chromatography with protein G-agarose (Fast Flow column; Millipore Co., Billerica, MA) and was quantified using an enzyme-linked immunosorbent assay quantitation kit for rat IgG2b antibody (Bethyl Laboratories, Inc., Montgomery, TX).

Techniques: Infection, Virus, Control

Journal: Journal of Virology

Article Title: Neutrophils Play an Essential Role in Cooperation with Antibody in both Protection against and Recovery from Pulmonary Infection with Influenza Virus in Mice

doi: 10.1128/jvi.01210-07

Figure Lengend Snippet: FIG. 5. Counts of total cells and PMN infiltrating and accumulating in the lungs of rat IgG-treated control mice (A) and MAb RB6-treated mice (B) with or without passive transfer of anti-PR8 antiserum or NMS on day 1 after virus infection (1.0 103 PFU). Open symbols, total-cell counts; solid symbols, PMN counts; triangles, anti-PR8; circles, NMS. Results are means from three experiments for 3 mice per group.

Article Snippet: The rat IgG2b MAb RB6 was prepared from the culture supernatant and ascites of pristane-primed BALB/c nude mice transplanted with the hybridoma by affinity chromatography with protein G-agarose (Fast Flow column; Millipore Co., Billerica, MA) and was quantified using an enzyme-linked immunosorbent assay quantitation kit for rat IgG2b antibody (Bethyl Laboratories, Inc., Montgomery, TX).

Techniques: Control, Virus, Infection